ISHS Acta Horticulturae 850: III International Symposium on Saffron: Forthcoming Challenges in Cultivation, Research and Economics
A METHOD TO IMPROVE THE INDUCTION OF CALLUS FROM SAFFRON (CROCUS SATIVUS LINNEO) CORMS |
| Authors: | I. Suárez-Ambriz, M. González-Ronquillo, A. Domínguez-López, A. Trejo-González, L. Riverón-Negrete |
| Keywords: | daughter saffron corms, in vitro tissue culture, plant cell culture, plant growth regulators, in vitro contamination reduction, Iridaceae |
| DOI: | 10.17660/ActaHortic.2010.850.14 |
| Abstract:
In vitro callus and suspended cells induction’s response was compared from saffron corms (Crocus sativus L.) from a Spanish cultivar; some corms were immediately cultivated in vitro in November 2006 and the others were preserved in distilled water in laboratory until daughter corms formed and in vitro cultured in May 2007. For the explants of the mother corms, the medium used was Murashige-Skoog (MS), with 2-4 dichlorophenoxy acetic acid (2,4-D, 0, 1, 2 and 4 mg.L-1) and kinetin (Kin., 0, 1 mg.L-1) in liquid medium and in agar gelified medium. The explants of daughter corms had the same treatments and they were planted in MS medium with Naphtalene Acetic Acid (NAA, 0, 1, 2 and 4 mg.L-1) and Kin (0, 1 mg.L-1), as well in gel as liquid media. In mother corms in gel the average induction time was 31.6 days with 24% induction frequency and 85% contamination rate; in liquid medium the contamination reached 100%. In the case of daughter corms obtained in laboratory, treatments in gel had an average induction time of 12.4 days and 100% induction frequency and 15% contamination; in liquid treatments the appearance time of free cells varied from 10 to 14 days with 100% induction frequency and 25% contamination rate; there was no significant difference (P>0.1) among the different combinations with auxins, in gel as well as liquid media, in whose formulae there was always callus or free cells, whereas in the case of the media with kinetin only and witnesses there was no response at all. However, there was significant difference (P<0.045) between treatments in gel and those of the liquid medium. Additionally, whole daughter corms were planted both in gel and liquid media with NAA 2 mg.L-1 and Kin 1 mg.L-1, where induction of callus in gel medium was observed as well above liquid medium level and suspended cells within liquid medium. | |
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