|Authors: ||L. Landi, E. Costantini, E. Montironi, B. Mezzetti|
|Keywords: ||Fragaria × ananassa, genetic transformation, arbuscular mycorrhizal, PCR|
A powerful tool for manipulating plant endogenous phyto-hormones is the transformation with oncogenes originating from Agrobacterium tumefaciens or A. rhizogenes. The potential use of the rolC gene in improving strawberry cultivation can be assessed by a proper evaluation of the modifications induced by the expression of the gene on plant phenotype and as a consequence on their adaptability, production and quality.
The root-arbuscular mycorrhizal fungi (AMF) symbiosis is considered an interesting model for studying the modification induced by rolC gene in plant and in particular also the potential risk of plant – mycorrhizal transgene exchange.
Field trials with control and transgenic lines were carried out, and plant production and fruit quality parameters were observed.
Mycorrhizal infection with Trypan Blue (TB) staining was monitored on roots.
Results on plant production and quality indicate a positive effect of the rolC depending on the gene copy number.
Control and rolC plants showed a similar intensity of AMF infection.
The main factor inducing change of control and rolC plants, sampled at the vegetative and productive phases in plant symbiosis were the seasons.
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