ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 839: I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008

CLONING AND CHARACTERIZATION OF VvChiF III GENE FROM VITIS VINIFERA CV. 'FLAME SEEDLESS'

Authors:   A. Ebadi, S.A. Salami, M.R. Naghavi, I.B. Dry
Keywords:   acidic chitinases, fungal disease, resistance, PR proteins
DOI:   10.17660/ActaHortic.2009.839.90
Abstract:
A class III chitinase gene (VvChiF III) was isolated from genomic DNA of Vitis vinifera cv. ‘Flame seedless’. The VvChiF III open reading frame comprised 894 nucleotides with no introns and encoded a protein of 297 amino acids. Pairwise alignment through BLAST indicated that VvChiF III is located on chromosome 16. The amino acid sequence encoded by VvChiF III showed a high identity to that of a class III chitinase isolated from V. vinifera cv. ‘Koshu’ and to other acidic chitinase. A number of single nucleotide polymorphisms (SNPs) were identified between different class III chitinase genes. Analysis of the VvChiF III amino acid sequence showed that this gene corresponds to the Glyco-hydro-18 super family that consists of a signal peptide with the length of 25 amino acids.

Download Adobe Acrobat Reader (free software to read PDF files)

839_89     839     839_91

URL www.actahort.org      Hosted by KU Leuven      © ISHS