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ISHS Acta Horticulturae 839: I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008

FIRST EVIDENCE OF D-HPLC EFFICIENCY FOR AN AUTOMATED CDNA-AFLP IN THE APPLE SCAB RESISTANCE MODEL

Authors:   R. Paris, L. Dondini, S. Tartarini, S. Sansavini, D. Bastia, V. Mantovani, V. Gualdi, P. Piffanelli
Keywords:   cDNA-AFLP, d-HPLC, Malus domestica, Venturia inaequalis, resistance
DOI:   10.17660/ActaHortic.2009.839.62
Abstract:
Apple scab resistance is one of the most well-characterized plant-pathogen interactions in woody plant species. After the cloning of the apple scab resistance gene HcrVf2, we aimed at identifying the network of genes that are differentially expressed after pathogen challenge by cDNA-AFLP. As electrophoretic analyses are labour-intensive with only limited potential for automation and the recovery of DNA fragments from gels is cumbersome, we investigated the possibility of performing the cDNA-AFLP analysis by d-HPLC (denaturing high performance liquid chromatography) and automating the DNA fragments collection using the Transgenomic WAVE System. Direct sequencing of the collected DNA fragments was also carried out and our preliminary results are reported.

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