ISHS


Acta
Horticulturae
Home


Login
Logout
Status


Help

ISHS Home

ISHS Contact

Consultation
statistics
index


Search
 
ISHS Acta Horticulturae 836: XXIII International Eucarpia Symposium, Section Ornamentals: Colourful Breeding and Genetics

POLYMORPHIC ITS AS A TOOL TO IDENTIFY HYBRIDS AND THEIR PARENTS IN CULTIVATED GENISTEAE (FABACEAE)

Authors:   V. Malécot, N. Macquaire-Le Pocreau, G. Auvray, V. Kapusta
Keywords:   Cytisus, Genista, Teline, Internal Transcribed Spacer, hybridisation events
DOI:   10.17660/ActaHortic.2009.836.12
Abstract:
Internal Transcribed Spacer (ITS) of ribosomal DNA is a classical sequence used for phylogenetic analyses. Usually, concerted evolution homogenizes the numerous ITS of an organism in such a way that a single sequence is amplified. However, for vegetatively propagated plants of hybrid origin, several ITS sequences can be obtained from a single individual. By cloning ITS sequences of various taxa assigned to tribe Genisteae within Fabaceae, we have been able to show such ITS polymorphism in Cytisus × kewensis, C. × dallimorei, C. × praecox, Genista × spachiana and G. ‘Porlock’ among others. For these taxa, the distinct ITS sequences can be compared with sequences of putative parents found in literature. We have then confirmed that Cytisus × kewensis is a C. ardoinoi × C. multiflorus cross. We have also confirmed that Genista ‘Porlock’ is a cross between G. monspessulana and G. × spachiana. Genista × spachiana, commonly presented as G. canariensis × G. stenopetala cross may in fact be a more complex hybrid involving also G. monspessulana.

Download Adobe Acrobat Reader (free software to read PDF files)

836_11     836     836_13

URL www.actahort.org      Hosted by KU Leuven      © ISHS