ISHS Acta Horticulturae 834: III International Late Blight Conference
MICROSATELLITE GENOTYPIC ANALYSIS OF PHYTOPHTHORA INFESTANS IN CHINA |
| Authors: | Zhi-Hui Yang, Jie-Hua Zhu, Jiang-Wei Shen, Guo-Sheng Yao, Rui Liu |
| Keywords: | Phytophthora infestans, genetic diversity, SSR genotype, co-dominate molecular marker |
| Abstract:
With the two microsatellites, Pi4B and Pi4G, a total of twelve SSR genotypes of Phytophthora infestans were identified among the 271 isolates collected in China from 1996 to 2007. The SSR genotypes of P. infestans collected from potato were entirely distinctive from those from tomato. Among isolates collected from potato, 10 different SSR genotypes were detected, of which two (F-01 and F-06) dominated the population. From tomato, only 2 SSR genotypes were found, of which one genotype (B-03) formed the major proportion of the population. A total of five (D-03, F-01, F-05, F-06 and G-02) and six (F-01, F-05, F-06, F-07, G-02 and I-01) SSR genotypes were separately found among 175 and 81 isolates from potato in both Hebei and Heilongjiang provinces, respectively, of which F-01 dominated the population; however, four genotypes (D-03, D-05, F-01 and H-01) were detected among 6 isolates from potato in Yunnan province. Statistical analysis of allele frequencies generated by the two loci (Pi4B and Pi4G) indicated that diversity produced by the locus Pi4B was higher than that of Pi4G. | |
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