ISHS Acta Horticulturae 826: I International Medicinal and Aromatic Plants Conference on Culinary Herbs
COMPARISON OF DNA MARKER TECHNIQUES FOR LAMIACEAE |
| Authors: | A.G. Ince, S. Elmasulu, A. Cinar, M. Karaca, A.N. Onus, K. Turgut |
| Keywords: | RAPD, DAMD-PCR, PCR-STS, mitochondria and chloroplast DNA markers |
| Abstract:
In conservation biology and genetic studies the use of molecular markers can illuminate issues of demographic history, population connectivity, breeding structure and introgression, and therefore provide input strategies for conservation, management and genetic studies of target organisms. In the present study we compared three types of molecular markers: randomly amplified polymorphic DNA (RAPD), directed amplification of minisatellite region DNA (DAMD-PCR) and polymerase chain reaction-sequence-tagged site (PCR-STS) markers to determine which of these markers provide repeatable and higher level polymorphism information content values on five different genus; Pholomis, Melissa, Origanum, Rosmarinus and Sideritis. Studies clearly indicated that PCR-STS markers of mitochondria and chloroplast were less polymorphic as indicated with their low polymorphism information content (PIC) values. On the other hand the PIC values of DAMD-PCR and RAPD techniques were equally similar indicating that these two types of DNA markers are equally effective in genetic studies. However the numbers of amplicons were lower in DAMD-PCR than that of the RAPD-PCR. Although PCR-STS showed lower polymorphism, these markers could be extremely valuable in identification of naturally occurring inter-specific hybrids in some species of Lamiaceae. | |
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