ISHS Acta Horticulturae 826: I International Medicinal and Aromatic Plants Conference on Culinary Herbs
PROTECTIVE EFFECT OF TAMARIND SEED COAT EXTRACT AGAINST OXIDATION OF HUMAN LOW-DENSITY LIPOPROTEIN |
| Authors: | M. Suksomtip, S. Pongsamart |
| Keywords: | tamarind seed coat extract, LDL oxidation, Tamarindus indica L., TBARS, conjugated diene, apoB carbonylation, fluorescent product |
| Abstract:
Methanolic extract from seed coat of Tamarindus indica L. so called variety “sour” was studied for the ability to inhibit Cu2+ induced human low density lipoprotein (hLDL) oxidation. hLDL (0.5 mg/ml) in phosphate buffered saline (pH 7.4) was incubated with 10 µM cupric sulfate and various concentrations of seed coat extract at 37°C for 24 hrs. The extract inhibited formation of conjugated dienes, fluorescent products, protein carbonylation and thiobarbituric acid reactive substances generated in LDL in a dose-dependent manner over 25-1,000 ng/ml extracts. The inhibition of conjugated dienes formation of 1,000 ng/ml extract was lower than that of 10 µM EDTA and 20 µg/ml ascorbic acid. Nevertheless, the inhibition on fluorescence formation of 1,000 ng/ml extract was comparable to 20 µg/ml ascorbic acid, but little lower than 10 µM EDTA. Increasing in carbonylation and TBARS were all inhibited with the extract. The extract 1,000 ng/ml showed higher inhibition on TBARS formation but lower capacity to inhibit carbonyl formation than 1,000 ng/ml vitamin C. All results confirmed protective effect of the extract on Cu2+ induced oxidative modification of LDL in vitro. | |
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