ISHS Acta Horticulturae 825: I Balkan Symposium on Fruit Growing
THE RATE OF SHOOT REGENERATION FROM APPLE (MALUS DOMESTICA) LEAVES DEPENDING ON THE IN VITRO CULTURE CONDITIONS OF THE SOURCE PLANTS |
| Authors: | P. Gercheva, L. Nacheva, V. Dineva |
| Keywords: | plastic vessels, gas-permeating cover, auxin, regeneration |
| Abstract:
Efficiency of somatic organogenesis depends to a great degree on the physiological status of the in vitro source plants. Important factors are the cultivation conditions – nutrient media, cultivation plates, gas-exchange possibilities, temperature and light regime. The aim of the present study was to investigate the effect of conditions for cultivating the source plants (cultivation plates and nutrient media) on the regeneration capacity of the apple (Malus domestica) leaf explants. Studies were carried out with in vitro propagated plants of the apple cultivar ‘Chadel’. The source microplants were cultivated on MS nutrient media with the addition of sucrose 10 g/L and modified MS with added ВАP 2.5 µM, IAA 0.0571 µM and sucrose 30 g/L. Two types of cultivation plates were used in the experiment – glass jars and plastic vessels with a gas-permeating cover. Leaf segments of the source plants that had been cultivated in each of the four combinations of nutrient media/cultivation plates were set for regeneration. The nutrient media for regeneration were based on MS with added TDZ (7.5 µM), sucrose 10 g/L, sorbitol 30 g/L and different IAA content – 5, 10, 15 and 20 µM. The explants were cultivated in darkness for 15 days, after which – at a photoperiod of 16/8 hours (40 µmol m-2 s-1 PAR) at a temperature of 22±2°С for 25 days. The best results (over 80% regeneration) were achieved when using explants of plants grown in plastic vessels with a gas-permeating cover in modified MS nutrient medium. | |
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