ISHS Acta Horticulturae 825: I Balkan Symposium on Fruit Growing
IN VIVO ADAPTATION AND FURTHER DEVELOPMENT OF MICROPROPAGATED PEAR CULTIVARS |
| Authors: | K.M. Kornova, S.K. Popov |
| Keywords: | pear cultivars, micropropagation, adaptation, cultivation, growth |
| Abstract:
During the period of multiplication and rooting, the in vitro propagated plants grow under controlled temperature, light intensity and photoperiod. When taken out and transferred to ex vitro conditions the plants are exposed to some stress factors the more important of them being the change in the nutritional medium, the higher light intensity and the lower air humidity. On the other hand, the in vitro environment does not always provide optimum root formation for all the cultivars. The major aim of the present experiment was to study the possibilities of increasing the percentage of plant survival, as well as to induce a good vegetative growth of the microplants of two different pear cultivars transferred under non-sterile conditions. Their successful adaptation to in vivo conditions and further optimal development and cultivation represent a prerequisite for the production of a greater amount of authentic, certified and own-rooted planting material. ‘Beurré Giffard’ and ‘Packham's Triumph’ pear cultivars were used in the study. Various methods of planting the rootless microplants were tested, involving treating the stem basis with IBA solution in the presence and in the absence of callus structure. The percentage of survival after planting in turf-perlite substrate, as well as the height increment increase and thickness during their cultivation were studied. It was proven that the best way for inducing rhizogenesis for the non-rooted in vitro plants was removing the developed callus structure in the stem basis and then dipping the plants in an IBA solution. Plants that had been planted with their callus structure had lower survival rate but later they had more vigorous vegetative growth as far as height and thickness of the stem were concerned. | |
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