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ISHS Acta Horticulturae 825: I Balkan Symposium on Fruit Growing
EFFECT OF THE IN VITRO CONTAINER TYPE ON GROWTH CHARACTERISTICS OF THE MICROPLANTS IN IN VITRO PROPAGATION OF GF 677
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| Authors: | K.M. Kornova, S.K. Popov |
| Keywords: | vitro container, growth characteristics, in vitro multiplication, elongation, rooting |
Abstract:
Throughout the in vitro cultivation period the propagated plants grow under controlled temperature, light intensity and photoperiod.
That is why the vitro containers, in which they are placed, have a significant effect on the development of the microplants.
Light intensity reaching the microplants is of great importance as well as the air exchange in the growth chamber.
The aim of the experiment was to study the growth characteristics of the microplants of the peach rootstock GF 677 cultivated in different vitro containers for the purpose of optimizing the technological parameters at the different stages of the propagation process.
At the stages of multiplication, elongation and rooting the plants were grown in three different containers: jars with glass covers (control), square vitro vent containers with covers made of polypropylene enabling the gas exchange with the ambient air and oval vessels of polypropylene, hermetically sealed and having a breathing strip.
The best development of the microplants expressed in homogeneity, actively growing vegetative tip, stem height and proliferation conditions was established when growing them in square vitro vent containers and glass jars.
Compared to the oval and glass containers the square ones enable the cultivation of a bigger number of plants on the growing chamber shelves.
The use of new types of vitro containers is a prerequisite for increasing the number of the produced plants along with improving their growth characteristics.
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