ISHS Acta Horticulturae 825: I Balkan Symposium on Fruit Growing
MICROPROPAGATION OF GISELA 5 (CHERRY DWARF ROOTSTOCK): THE EFFECT OF THE TYPE AND THE CONCENTRATION OF THE CARBOHYDRATES IN THE NUTRIENT MEDIUM |
| Authors: | L. Nacheva, P. Gercheva |
| Keywords: | sucrose, sorbitol, micropropagation, Gisela 5 |
| Abstract:
Gisela 5 Cherry Dwarf Rootstock has a moderate to poor growth and it is very perspective for the development of modern intensive cherry plantations. There are single announcements in scientific literature about micropropagation of Gisela 5. The aim of the present study was to investigate the effect of the type and the concentration of carbohydrates (sucrose and sorbitol) added to the nutrient medium with auxins (IBA, NAA and IAA) on the multiplication coefficient and the length of the newly formed shootlets in Gisela 5 Cherry Dwarf Rootstock. The study was carried out on 15 different nutrient media suitable for multiplication, based on MS (1962). The effect of the varying concentrations of auxins (IBA, NAA and IAA) and carbohydrates (sucrose and sorbitol used separately and in a combination) at a constant concentration of the cytokinin BAP (2.5 µM), was followed out. The results of the investigations showed that the basic effect on both studied characteristics was exerted by the type and the concentration of the carbohydrates. The highest multiplication coefficient (number of shootlets per plant) was achieved when combining sucrose and sorbitol in a 2:1 ratio, followed by the variants of sucrose to sorbitol in a 1:2 ratio. The most distinct differences were reported in the variants on the nutrient medium with IBA and IAA when the coefficient of multiplication was 3-4 times higher compared to the classical variants with sucrose. The best results (multiplication coefficient of 3.72-4.31 at 1.9 cm mean length of the shootlets) were achieved in the variants containing 0.005 µM IBA, 20 g/L of sucrose and 10 g/L of sorbitol. | |
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