ISHS Acta Horticulturae 823: XI International Symposium on the Processing Tomato
DEVELOPMENT AND STANDARDISATION OF AN ACCELERATED SOLVENT EXTRACTION METHOD FOR LYCOPENE ANALYSIS |
| Authors: | L. Sandei, P. Risi, F. Bloise |
| Keywords: | tomato, ASE (accelerated solvent extraction), analysis, DAD HPLC |
| Abstract:
Consumers, researchers and marketing people in the food industry have become considerably aware of the health benefits of lycopene and of the other carotenoids from tomatoes; as is well known, tomato products provide the greatest source of these important phytonutrients with strong antioxidant activity. The SSICA tomato department has developed a new standard method for the accelerated extraction and determination of lycopene in tomato products (fresh matter, juices, purees, pulp, concentrates and sauces). This is an accessible, rapid and reproducible analysis utilizing ASE equipment (accelerated solvent extractor) with THF (tetra hydro furan) solvent in proper conditions. For the validation of this method and for the quantitative determination, an HPLC (Column C18 - Diode Array Detector) system was used which is currently in use in the SSICA tomato research lab. This method enables a rapid, precise and reproducible analysis and will be useful in terms of solvent and time saving and high automatism with high precision results. | |
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