ISHS Acta Horticulturae 822: VI International Pineapple Symposium
CHARACTERIZATION AND MANAGEMENT OF PINEAPPLE MEALYBUG WILT ASSOCIATED VIRUSES |
| Authors: | J.S. Hu, D.M. Sether, M.J. Melzer, C.V. Subere, K. Cheah, Y. Chen, Qi Li, W. Borth, I.C. Wang, C. Nagai, M.L. Wang |
| Keywords: | virus diversity, vector transmission, etiology, control |
| Abstract:
Molecular diversity of ‘Pineapple mealybug wilt associated viruses’ and badnavirus-like viruses were investigated. Phylogenetic analyses placed PMWaV-1, PMWaV-3, PMWaV-4, PBNSPaV, and some GLRaVs in a distinct clade within the genus Ampelovirus. Cloning and sequencing of amplicons of badnavirus-like sequences revealed the presence of at least four different clades, designated A, B, C, and D. The phylogenetic relatedness of clades A, B, C, and D to other badnaviruses, other Caulimoviridae members, and retro-like elements, and the distribution of the clades were examined. Vector transmission characteristics of PMWaV-2 including acquisition access period (AAP), persistence and retention of infectivity of the virus in grey pineapple mealybug vectors were evaluated. PMWaV-2 is transmitted by the grey pineapple mealybug in a semi-persistent manner. RT-PCR assays also showed that longtailed mealybug can acquire and transmit PMWaV-2, although not as efficiently as the grey pineapple mealybug. Approaches using non-transgenic and transgenic methods to control ‘Pineapple mealybug wilt-associated viruses’ and badnaviruses were evaluated. | |
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