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ISHS Acta Horticulturae 818: I International Symposium on Pomegranate and Minor Mediterranean Fruits

ANTIMICROBIAL ACTIVITY OF CRUDE EXTRACTS FROM POMEGRANATE (PUNICA GRANATUM L.)

Authors:   L. Salgado, P. Melgarejo, I. Meseguer, M. Sánchez
Keywords:   plant extract, pomegranate, antimicrobial
Abstract:
In the vegetable kingdom there are many substances showing antimicrobial properties. Though these secondary compounds are not indispensable for vegetables, they increase plant survival possibilities. Those can be used as therapeutic agents, additives, alimentary preservatives or biological agents for agricultural diseases. In fact, pomegranate (Punica granatum L.) is a fruit tree species that shows very low bacterial disease incidence. The current study analyses and evaluates the antimicrobial effectiveness of pomegranate fruit raw extracts over the following bacterial species: Pseudomonas aeruginosa, Escherichia coli, Enteroccocus faecalis, Enterobacter aerogenes, Staphyloccocus aureus, Microccocus luteus and Bacillus sp. Our results shows that all the crude extracts from P. granatum fruit posses antimicrobial activity although, at the concentrations tested, none was more effective than a solution containing 1% phenol. According to the data obtained, the most powerful extract resulted to be the one from the pomegranate juice. The antimicrobial effectiveness of the extracts also depends on the species of bacteria evaluated, the more sensitive being the Gram-positive species Staphyloccocus aureus and Bacillus sp. Our next aim will be the identification and purification of the juice substances that shows this antimicrobial activity.

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