ISHS Acta Horticulturae 818: I International Symposium on Pomegranate and Minor Mediterranean Fruits
EVALUATION OF RAPD MARKERS FOR ASSESSMENT OF GENETIC VARIATION AMONG POMEGRANATE GENOTYPES |
| Authors: | Z. Zamani, A. Sarkhosh, M.R. Fatahi, A. Ebadi |
| Keywords: | Punica granatum, genetic diversity, molecular markers, UPGMA, PCR |
| Abstract:
Pomegranate (Punica granatum L.) is grown in Iran from ancient times and a high level of diversity of this fruit is present in this country. Clear identification of pomegranate genotypes is a valuable task for different purposes, especially for breeding works. RAPD markers were used to study 24 genotypes, some of them having similar names but collected from two places. DNA was extracted from leaf samples and a number of 113 decamer primers were tested on them, among which 27 showed good amplification and produced 158 polymorphic bands. Cluster analysis using Jaccard’s similarity coefficient and UPGMA method divided these genotypes into 8 sub-clusters at a distance of 0.64 on the dendrogram. Genotypes with similar names did not show a high similarity and scattered into different subclusters. Geographic origin of genotypes affected the clustering to a high extent, while characteristics such as fruit color or taste did not show prominent effect. The observed variation proved the richness of pomegranate genetic resources in Iran. Also RAPD markers performed as a suitable technique for pomegranate genetic studies. | |
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