ISHS Acta Horticulturae 815: International Workshop on Chestnut Management in Mediterranean Countries - Problems and Prospects
DETERMINATION OF TOTAL PHENOLIC COMPOUNDS IN CULTIVARS OF CASTANEA SATIVA MILL. |
| Authors: | I. Mujić, S. Dudaš, J. Živković, J. Tomin, Z. Zeković, V. Alibabić, H. Jukić, R. Šahinović |
| Keywords: | Cultivar, dry extract, extragent, natural antioxidants, sweet chestnut |
| Abstract:
The cultivar of the sweet chestnut, Lovran's ‘Marrone’ and grafted Italian ‘Marrone’ have been investigated in this study. The analysis were done with different parts of sweet chestnut, such as: peeled seed, brown shell, red seed coat, seed, leaf, catkin, spiny bur, as well as the new and old chestnut bark, which were extracted by 50% ethanol, as well as by 50% acetone, as an extragents. The plant material was milled to an average particle size of 0.18 to 0.40 mm. The yield of dry extract, expressed as % (w/w), measured from 1.82% for spiny burs (extragent 50% ethanol) to 33.56% for catkins (extragent 50% acetone). The total phenolic compounds content obtained in dry extracts was determined using the Folin – Ciocalteu assay and expressed as g of gallic acid equivalents (GAE) per 100 g of the dry extract sample (%; w/w), i.e. % gallic acid equivalents (GAE). That value was from 0.42 %GAE for the seed (extragent 50% ethanol) to 6.89 %GAE for the leaf (extragent 50% acetone). The lowest content of %GAE (0.11) were found on peeled seed (extragent 50% ethanol), and the highest (11.42 %GAE) on catkins (extragent 50% acetone). The %GAE value of grafted Italian "Marrone" cultivar was the lowest (0.41 %GAE) for the sample of the whole fruit (extragent 50% ethanol) and the highest (6.45 %GAE) has obtained for the leaf (extragent 50% acetone). The studied sweet chestnut cultivars are important natural antioxidants for use as food ingredients. | |
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