ISHS Acta Horticulturae 814: XII EUCARPIA Symposium on Fruit Breeding and Genetics
TOWARD THE ISOLATION OF THE D GENE CONTROLLING THE ACIDITY OF PEACH FRUIT BY POSITIONAL CLONING |
| Authors: | K. Boudehri, M.A. Belka, G. Cardinet, G. Capdeville, C. Renaud, Y. Tauzin, E. Dirlewanger, C. Troadec, D. Jublot, M. Caboche, A. Bendahmane, A. Moing |
| Keywords: | BSA-AFLP, BAC library, fruit quality, peach |
| Abstract:
The sugar/acid ratio is an essential component of the organoleptic quality for fruits in the Rosaceae family. The D gene, controlling the non-acid trait in peach, is dominant and segregates as a Mendelian character. Using a mapping progeny of 208 F2 individuals derived from a cross between two peach varieties ‘Ferjalou Jalousia®‘ and ‘Fantasia’, the D gene was mapped on linkage group 5. Using the BSA-AFLP method with 960 primer combinations, 12 AFLP markers were identified within 10 cM around the D gene. Two of them co-segregate with the D gene. The fine mapping of the region around the D gene, based on the analyses of an extended F2 progeny of 1510 seedlings, is in progress. Three AFLPs were transformed into codominant SCARs and will be used to screen a new BAC library obtained from the F1 hybrid that is the parent of the F2 progeny. Screening of the BAC library with the flanking markers of the D gene will allow the identification of the clones containing the D and d alleles. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
