ISHS Acta Horticulturae 814: XII EUCARPIA Symposium on Fruit Breeding and Genetics
STUDIES ON MRNA EXPRESSION OF GENES INVOLVED IN FLORAL MERISTEM TRANSITION OF APPLE (MALUS DOMESTICA BORKH) |
| Authors: | C. Hättasch, D. Kapturska, H. Flachowsky, F. Dunemann, M.-V. Hanke |
| Keywords: | Malus domestica, flowering genes, floral induction, transition, MdCOL, MdSOC1, MdFT, MdTFL1, AFL, MdMADS2, MdMADS5 |
| Abstract:
While the phenotypic development of apple flowers is well understood, the genetic mechanism of floral induction and flower development are widely unclear. In this study we isolated the native flowering genes MdCOL1 and MdCOL2 from genomic DNA of the apple ‘Pinova’ and identified until now unknown intron sequences of MdCOL1 and MdCOL2. In addition, we isolated the MdTFL1-2 gene from genomic DNA of ‘Pinova’. In DNA blot analyses, we found at least two strong bands for MdCOL, MdSOC1, MdFT, AFL, MdMADS2, MdMADS5, and MdTFL1. First expression analyses of the mRNA levels showed that the floral suppressor gene MdTFL1 gave response to the full bloom stage in vegetative shoot meristems and was repressed just before floral differentiation. In contrast, the flower inducing genes MdCOL1 and MdCOL2 were neither activated in vegetative nor in reproductive shoot meristems. | |
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