ISHS Acta Horticulturae 814: XII EUCARPIA Symposium on Fruit Breeding and Genetics
S-ALLELE IDENTIFICATION IN JAPANESE PLUM CULTIVARS BY PCR AND CROSS-POLLINATION |
| Authors: | M.E. Guerra, J. Rodrigo, M. López-Corrales, A. Wünsch |
| Keywords: | Japanese plum, Prunus salicina, S-alleles, self-incompatibility, incompatibility group, pollen tube growth |
| Abstract:
Japanese plum exhibits gametophytic self-incompatibility. This mechanism is genetically determined by a locus (S) with multiple alleles. This locus encodes an allele-specific S-RNase, expressed in the style, which inhibits the growth of pollen tubes with the same S alleles. Since most Japanese plum cultivars are self-incompatible, it is necessary to use pollinator cultivars to obtain fruit set. In this work, the S-alleles of 38 Japanese plum cultivars were determined by PCR amplification of the S-RNase gene. These include 24 cultivars whose S-alleles have not been described before. The results allowed grouping the cultivars in 11 Incompatibility Groups, of which 4 are new, and the identification of a new S allele, So. Observation of pollen tube growth under the microscope in self- and cross-pollinations allowed confirming the Incompatibility Groups determined by PCR. | |
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