ISHS Acta Horticulturae 814: XII EUCARPIA Symposium on Fruit Breeding and Genetics
BULKED SEGREGANT ANALYSIS FOR THE IDENTIFICATION OF MOLECULAR MARKERS LINKED TO SELF-COMPATIBILITY IN 'CRISTOBALINA' SWEET CHERRY |
| Authors: | A.M. Cachi, A. Wünsch |
| Keywords: | gametophytic self-incompatibility, self-compatibility, sweet cherry, Prunus avium, BSA, bulked segregant analysis, AFLP |
| Abstract:
Self-compatibility in a naturally self-incompatible species like sweet cherry is a highly interesting trait for breeding purposes. ‘Cristobalina’ is a self-compatible local Spanish sweet cherry cultivar. Preliminary studies on this cultivar suggest that self-compatibility is caused by factors located outside the S locus. In this work, self-compatibility was determined in a segregating progeny by using fluorescence microscopy. The resulting self-compatible and self-incompatible genotypes were used to construct DNA bulks that were used to search for markers linked to the self-compatibility locus by Bulked Segregant Analysis. Eighty-eight SSR loci, derived from apple and peach, and 256 AFLP primer combinations were screened in two DNA bulks of eight self-compatible and eight self-incompatible progeny genotypes. Polymorphic markers among the bulks were analysed in the parental genotypes and finally in the rest of the population to confirm their linkage to the self-compatibility locus. | |
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