ISHS Acta Horticulturae 814: XII EUCARPIA Symposium on Fruit Breeding and Genetics
IDENTIFICATION OF S-ALLELES IN SEVERAL APPLE CULTIVARS |
| Authors: | K. Bokszczanin, A. Palucha, A.A. Przybyla |
| Keywords: | Malus x domestica Borkh., S-allele, RT-PCR |
| Abstract:
Two Polish apple cultivars ‘Ligol’ and ‘Odra’ and two Polish apple clones ‘U633’ and ‘U1065’ were utilized for studies of self-incompatibility. PCR amplification using FTC143 and FTC144 S-allele specific primers for S7 failed to differentiate between the S7 and Sz alleles. Based on available sequence data, a new set of primers upstream and downstream of the hypervariable region of apple S-RNases was designed. Using the RT-PCR method, cDNA was amplified on RNA isolated from styles of selected plants. PCR products were cloned and sequenced. In cultivar ‘Odra’ and clones ‘U633’ and ‘U1065’, a new S-allele, showing 97% of identity with Sauc19 derived from Sorbus aucuparia (EF494758) and denoted as a S-kb, was identified. The Sz allele was associated with cultivar ‘Odra’, and S7 with ‘U1065’. In ‘Ligol’, the agronomically most important cultivar in Poland, S2 and S9 are present. | |
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