ISHS Acta Horticulturae 814: XII EUCARPIA Symposium on Fruit Breeding and Genetics
SOME RESULTS OF STUDY CONCERNING IN VITRO TECHNIQUES OF BREEDING AND IMPROVEMENT OF PRUNUS DOMESTICA L. |
| Authors: | A. Barbulescu, M. Neamtu, A. Ilie, A. Petcu, L. Ion, G. Popa |
| Keywords: | Prunus domestica ¿Centenar¿, in vitro propagation, shoot tip |
| Abstract:
The aim of this study was to determine an effective propagation protocol for Prunus domestica ‘Centenar’. Shoot tip was cultivated in vitro on a Murashige-Skoog (MS) medium containing 1.0 mg/L BA, 0.2 mg/L NAA, 30 g/L sucrose and 6 g/L agar, pH 5.6. Explants were subcultured at three-week intervals on the same medium. A concentration of 1.0 mg/L IBA in half strength of MS provided the best rooting. After acclimatization, the regenerated plantlets were transferred to soil under natural conditions. | |
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