ISHS Acta Horticulturae 814: XII EUCARPIA Symposium on Fruit Breeding and Genetics
COMPARATIVE ANALYSIS OF GENETIC DIVERSITY IN TUNISIAN DATE-PALM (PHOENIX DACTYLIFERA L.) AS REVEALED BY RAPDS AND AFLPS |
| Authors: | S. Rhouma, A. Ould Mohamed Salem, S. Zehdi-Azouzi, K. Chatti, A. Rhouma, M. Marrakchi, M. Trifi |
| Keywords: | AFLPs, RAPDs, comparison, correlation, Phoenix dactylifera, relationships |
| Abstract:
The genetic diversity organization and evaluation of relationships within Tunisian date-palm (Phoenix dactylifera L.) cultivars were assessed using the Random Amplified polymorphic DNA (RAPD) and the Amplified Fragment Length Polymorphism (AFLP) methods. Four random universal primers and six AFLP primers combinations were tested with DNAs isolated from twenty-one cultivars in order to compare the efficiency of these techniques to generate molecular polymorphisms. As a result, all tested primers have permitted to generate large number of molecular markers that are suitable to survey genetic diversity in this crop. Therefore, a large and typically continuous genetic diversity characterizes the local date-palm germplasm. In addition, the UPGMA dendrogram based on combined AFLP and RAPD data exhibited a topology nearly similar to the AFLPs-based one, suggesting that AFLP is more efficient for the establishment of genetic relationships among date-palms than the RAPD method. This assumption is strongly supported since low but significant correlation between RAPD and AFLP has been scored using the Mantel test. The opportunity of the designed methods is discussed with the molecular characterization of genotypes in order to enhance the conservation and the improvement of the local germplasm. | |
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