ISHS Acta Horticulturae 813: VI International Symposium on New Floricultural Crops
PROPAGATION TECHNIQUES FOR IBERIS SEMPERFLORENS L. |
| Authors: | G. Iapichino, M. Bertolino |
| Keywords: | Germination, cutting propagation, micropropagation, Mediterranean flora |
| Abstract:
Recently, many plants native to the Mediterranean region are sought and selected for their ornamental value. Iberis semperflorens L. is an evergreen subshrub (30-60 cm) endemic to southern Italy and Sicily with attracting white fragrant flowers in corymbs which flowers from October to April. Although I. semperflorens has potential for pot cultivation and outdoor ornamental use there is a lack of detailed and published research results on the propagation of this winter flowering species. The aim of this research was to define commercially suitable propagation methods (seed, cuttings, tissue culture) for I. semperflorens by studying the influence of temperature on germination. Seeds were germinated at various constant temperatures (14, 20, 24 and 28°C) either in continuous light or darkness. Highest germination occurred at 20°C in light. To study the influence of temperature and auxin treatment on vegetative propagation, cuttings were inserted in a mixture of peat and perlite with or without bottom heat and either dipped in a 500 mg/L 1H-indole-3-butyric acid (IBA) solution or an untreated control. Rooting was markedly improved by bottom heat (22 ± 2°C) and IBA treatment, whereas almost no rooting was observed without bottom heat. Highest in vitro axillary shoot proliferation was induced on Murashige and Skoog (MS) agar medium supplemented with 1.78 μM benzyladenine (BA). Microshoots rooted after four weeks on MS agar medium augmented with 2.85 μM indole-3-acetic acid (IAA). | |
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