ISHS Acta Horticulturae 813: VI International Symposium on New Floricultural Crops
GENETIC VARIABILITY IN CATTLEYA VIOLACEA (ORCHIDACEAE) IN THE AMAZONIAN REGION |
| Authors: | Elizanilda Ramalho do Rego, Mailson Monteiro do Ręgo, Bruno de Campos Souza |
| Keywords: | genetic diversity, RAPD markers, genetic resources |
| Abstract:
The variability within the Orchidaceae family is perceived in a large number of genera and species, as well as in the diversity within the same species, as found in Cattleya violacea (H.B.K.) Rolfe. Few plants surpass the orchids in their distribution throughout the world, variation in growth habits, and the magnificent spectrum of colors produced by their flowers and leaves. Not surprisingly, flowers’ color variation is often linked to assemblages of a number of species and varieties. The aim of this work was to analyze the genetic variability among eleven C. violacea varieties from the Amazonian Region (three Brazil Amazonian States and one Venezuela Amazonian region) with different flower coloration: Cattleya violacea cv. caerulense, Cattleya violacea cv. maravilha, Cattleya violacea cv. semi-alba, Cattleya violacea cv. semi-alba estriata, Cattleya violacea cv. semi-alba flamea, Cattleya violacea cv. rubra, Cattleya violaceae cv. caerulea, Cattleya violacea cv. semi-alba fantasia, Cattleya violacea cv. amesiana, Cattleya violacea cv. bicolor and Cattleya violacea cv. alba. DNA was extracted by CTAB protocol and then submitted to amplification by RAPD analysis and separation of amplification products by agarose electrophoresis. The grouping was made by nearest neighbor method based on similarity matrix from Jaccard index complement. The varieties were arranged in ten different groups. The most similar varieties were Cattleya violaceae caerulense and Cattleya violacea caerulea that had blue flowers and were collected in Mato Grosso state and Roraima state. The most dissimilar were Cattleya violacea rubra and Cattleya violacea semi-alba estriata from the same region of Roraima state and they were similar with white and purple flower coloration. The genetic diversity analysis showed the necessity for conservation of the biologic diversity of the genus Cattleya and Orchidaceae family in Amazon Region. | |
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