ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
GERMINATION AND DEVELOPMENT OF SOME TERRESTRIAL ORCHIDS IN VITRO |
| Author: | G. Jakobsone |
| Keywords: | in vitro, wild orchids, development |
| Abstract:
Thirty-three species of the family Orchidaceae are growing in Latvian wild areas and 26 are included in the Red Data Book of Latvia. Two of them (Cypripedium calceolus L. and Liparis loeselii (L.) Rich.) are protected by Council Directive 92/43/EEC of 21 May 1992 on the conservation of natural habitats and of wild fauna and flora. The objective of our study was to carry out the conservation of rare and endangered orchids ex situ in two ways: by establishing an in vitro bank, and by supplementing the existing Orchidaceae collection of the National Botanic Garden. The results of our three year investigation are summarized with the in vitro germination of seven species out of the nine sowed: Cypripedium calceolus, Liparis loeselii, Gymnadenia conopsea (L.) R. Br. and four species of the genus Dactylorhiza: D. baltica (Klinge) N. I. Orlova, D. incarnata (L.) Soó, D. fuchsii (Druce) Soó and D. russowii (Klinge) Holub. Asymbiotic germination was achieved with seeds at the most suitable developmental stage for multiplication in vitro and with the optimal formula of the initial culture medium and cold storage (in some cases). In vitro culture allows observing all early stages of morphogenesis of orchids; under natural conditions these stages take place underground. Plants of Dactylorhyza fuchsii and Gymnadenia conopsea obtained in vitro were transplanted to the soil in order to further study their development ex vitro. | |
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