ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
STRATEGY TO IMPROVE THE QUALITY, ACCLIMATIZATION AND EX VITRO RE-INTRODUCTION OF MICROPROPAGATED PLANTS OF LIMONIUM CORDATUM (L.) MILL., A MEDITERRANEAN ENDEMIC |
| Authors: | M. Savona, C. Mascarello, E. Mantovani, L. Minuto, G. Casazza, B. Ruffoni |
| Keywords: | Photosynthetic Proton Flux Density (PPFD), temperature, rooting, alophylous, in situ conservation |
| Abstract:
Limonium cordatum is a halophilous plant which lives in a very restricted area on the coast of the Mediterranean. It is included in the regional Red Lists of threatened plants in the Liguria Region (I) and in the Dept. des Alpes Maritimes (F) (I.U.C.N., 1998). In order to implement a successful conservation strategy, the possibility to establish a protocol of in vitro micropropagation was explored. Shoots regenerated from immature flower stems resulted to be the best starting materials. Sterile shoots were cultured on an agar medium containing Murashige and Skoog medium (1962) salts and vitamins, 30 g L-1 sugar, supplemented with 6-BA 0.2 mg L-1 and IAA 0.5 mg L-1. Under these conditions it was possible to obtain a high multiplication rate but the short shoots produced were not suitable for rooting and acclimatization. To improve the quality of the micropropagated plants, we investigated the effect of 2 different light intensities and two temperature levels. With the lowest light intensity, it was possible to obtain rooted plants which could be successfully transferred to the greenhouse. Six-month old acclimatized plants were used in an in situ reintroduction programme. | |
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