ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
GENETIC STABILITY OF SEVERAL CULTIVARS OF GRAPEVINE CULTIVATED IN VITRO |
| Authors: | C. Crăciunaş, A. Coste, B. Oltean, M. Farago, M. Iliescu, R. Iuoras, A.L. Butiuc-Keul |
| Keywords: | grapevine, cultivars, micropropagation, RAPD |
| Abstract:
Vitis vinifera have been the subject of extensive genetic studies due to their worldwide cultivation and importance. In vitro micropropagation for different purposes could generate somaclonal variations. In order to preserve valuable grapevine cultivars in germplasm collections the genetic stability of in vitro plantlets should be tested. Molecular markers can provide valuable information about the genetic polymorphism that may appear during in vitro culture. The following cultivars were studied: ‘Italian Riesling’, ‘Muscat Ottonel’, ‘Brumariu’, ‘Radames’ and ‘Feteasca Regala’. Genetic conformity was studied by RAPD markers. Somaclonal variation selection by RAPD markers was not possible because the patterns of different in vitro plantlets belonging to the same cultivar were similar. RAPD markers obtained by DNA amplification with different primers showed no any genetic polymorphism in the population of the in vitro plants, but could discriminate the plantlets of different cultivars. In vitro micropropagation of those grapevine cultivars that have been studied, it is in fact a clonal propagation. | |
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