ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
ACCLIMATION AND ESTABLISHMENT OF CORK OAK (QUERCUS SUBER) SOMATIC EMBRYO-DERIVED PLANTLETS AND POST-ACCLIMATION CORK QUALITY TEST |
| Authors: | B. Pintos, J.A. Manzanera, M.A. Bueno, A. Cremades, J.R. González-Adrados |
| Keywords: | field trial, germination, maturation, progeny test, survival |
| Abstract:
Cork oak (Quercus suber) somatic embryos were cultured on basal medium with 3% sucrose and 1% activated charcoal for two months at 4°C and germinated on medium supplemented with 6-benzylaminopurine and indole-3-butyric acid. Plantlets were transferred to 2-L pots and acclimated in a nursery, evaluating the survival rate one year after transfer. Histology studies revealed that the cork (phellem) layer of somatic plantlets started periclinal divisions during the first year of age, as well as in the one year-old twigs of the parent trees. The percentage of virgin cork thickness in three and four year-old twigs of the parent trees showed statistical differences between trees of different stand locations. This phenotypic trait showed a moderately strong correlation between the parents and the progenies, which also showed significant differences between stand locations. Our results of 909 cork oak plantlets acclimated from somatic embryogenesis, with a high survival rate (78%), from which 507 were transferred to a field trial, prove that this methodology is applicable for large scale plantlet production and that the regenerated plantlets show a cork quality trait related to that of the parent tree. | |
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