ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
BACTERIAL CONTAMINATION IN ILEX DUMOSA (AQUIFOLIACEAE) CULTURES: ANTIBIOTIC TREATMENT |
| Authors: | C. Luna, M. Collavino, P. Sansberro, L. Mroginski |
| Keywords: | immersion temporal system, RITA, plant regeneration |
| Abstract:
A range of commercially antibiotics were tested on nodal segments-culture of Ilex dumosa for their ability to control bacterial (Gram negative) contamination. Thirteen treatment with 4 antibiotic were tested: streptomycin (200 mg.ml-1), chloramphenicol (50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg•ml-1) and combinations between ampicilline (200 mg.ml-1) + chloramphenicol (50 mg.ml-1), streptomycin (200 mg.ml-1) + cefotaxime (0.50 mg.ml-1), cefotaxime (0.25, 0.50 and 1 mg.ml-1) + ampicilline (200 mg.ml-1) or chloramphenicol (50 mg.ml-1). Nodal segments of I. dumosa were cultured in Murashige and Skoog (1962) medium, quarter strength (1/4MS), with 1.5% sucrose and 20 μM BAP in a RITA® immersion temporal systems (with a frequency of 1 min. each 4 h ) under 27±2°C with lighting (cool white fluorescent light) at 116 μmol•m-2•s-1 and 14 h photoperiod. After 30 days of culture various antibiotics controlled the proliferation of bacteria. The most suitable treatment was 0.50 mg.ml-1 cefotaxime which permitted 100% of establishment with 100% of explants with shoots with 8 cm in average. Rooting of regenerated shoots produced plants that were successfully transferred in pots. | |
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