ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
NITROGEN ASSIMILATING ENZYMES DURING ACCLIMATIZATION OF MICROPROPAGATED MUSA SP. PLANTS |
| Authors: | A. Martín-Bernabé, J.L. Casas, A. Piqueras |
| Keywords: | nitrate reductase, glutamine synthetase, NADH-GOGAT, Fd-GOGAT, stress, in vitro culture |
| Abstract:
Acclimatization of micropropagated plants to ex vitro conditions is an essential step in any micropropagation protocol. In this work we planned to follow the activity of the main enzymes involved in the assimilation of nitrogen in leaves and roots of micropropagated Musa sp. plants during acclimatization. We followed the activity of nitrate reductase (NR), glutamine synthetase (GS), NADH-dependent glutamate synthase (NADH-GOGAT) and Fd-dependent glutamate synthase (Fd-GOGAT) in leaves and roots weekly until the sixth week of acclimatization. The results support the view that acclimatization imposes a very distinct type of physiological relationships in the plants than during normal growth. This is revealed by the fact that roots and in vitro leaves showed a similar pattern along the period under study and highlight the role of the roots not only in the assimilation of nitrate but also in the utilization of nitrogen through GS-GOGAT cycle. This similarity between the two organs vanished with the appearance of newly ex vitro formed leaves. | |
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