ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
IN VITRO MULTIPLICATION, ROOTING, ACCLIMATIZATION AND RELATED PROTEIN PROFILES OF ROOTSTOCK 'CITATION' (PRUNUS SALICINA × P. PERSICA) |
| Authors: | C. Damiano, G. Delia, A. Frattarelli, L. Farina, M. Buccheri |
| Keywords: | 2-DE, culture media, micropropagation, proteomic, 'Zaipime' |
| Abstract:
The rootstock ‘Citation’ [‘Zaipime’] (Prunus salicina × P. persica) was obtained in USA by F. Zaiger. At present, in Italy, it is under evaluation as rootstock of plum and apricot, while it is no longer recommended for peach, because of the numerous cases of graft incompatibility. In the present work different aspects of micropropagation and correspondent expressed protein profiles are presented. The plants were multiplied onto Murashige and Skoog (1962) basal medium with different combinations of BA, GA3, NAA. Basal salts, IBA, activated charcoal, explants source and different concentrations of zinc sulphate heptahydrate were used to test their influence on rooting. Plants resulting from different culturing conditions showed a variability in multiplication rate and in rooting aptitude, ranging from 100% of the best rooting percentage to almost 0% of the controls (auxins-free). After 40 days the best acclimatization percentage (100%) was obtained with the rooting media MS macro salt half strength, MS micro elements full strength and IBA (0.5 mg/L), without activated charcoal. Plants rooted on the same medium, but with activated charcoal, showed less survival percentages better growth and development. To obtain a better knowledge of biology of the micropropagation steps, a proteomic approach has been undertaken. Total proteins content extracted from in vitro propagated material at different stages was separated through a 2-DE. Protein profiles were analyzed with Image Master software in order to identify differences between samples. The analysis carried out, so far, prove the existence of modification in protein pattern and concentration during micropropagation stages. These results may also represent some groundwork to the comprehension of graft incompatibility with peach. | |
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