ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
ACCLIMATIZATION OF SOMATIC HYBRIDS AND PLANTLETS AFTER CHROMOSOME DOUBLING IN SOLANUM GENUS |
| Authors: | H. Polzerová, M. Greplová |
| Keywords: | oryzalin, potato dihaploids, somatic hybrids, wild Solanum species |
| Abstract:
Many valuable resistance genes are carried by wild species of Solanum genus that are often non-crossable with cultivated potato. Somatic hybridization and mitotic polyploidization are approaches to overcome this problem. At our workplace many somatic hybrids were developed and their in vitro morphology was assessed. For further assessment of phenotype an evaluation should be done under in vivo conditions. Optimal method for the most effective system to transfer plantlets into in vivo conditions was investigated. Three systems of acclimatization (nursery tray, plastic and peat pots) were tested. A set of somatic hybrids of two combinations was planted into wells of nursery tray (3×3 cm) containing sterilized soil and cultured in a laboratory with artificial illumination (60 μmol m-2 s-1) of 16 h photoperiod at 22°C. The second variant involved direct planting into the plastic pots (5×5 cm). Plants planted in this way were partially protected by non-woven fleece and exposed to weather conditions. Rate of survival and growth increases were investigated in both systems. In vitro polyploidization was used for deriving of breeding material. Nodal segments of wild species of Solanum genus and S. tuberosum dihaploids were treated with mitotic poison. Ability of individual genotypes to manage stress during the polyploidization process, yielding capacity of derived doubled materials and their ability to acclimatize to in vivo conditions were studied. Doubled plantlets were planted into the wells of nursery tray and into peat pots (5×5 cm) both variants in the greenhouse and ability of individual genotypes to survive and to grow further were studied. Plants acclimatized in this way were then used to morphology assessment and in crossing with tetraploid S. tuberosum. Acclimatization through nursery tray was better in both somatic hybrids and doubled plants of Solanum genus. | |
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