ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
DEVELOPMENT, MATURATION AND GERMINATION OF HORSE CHESTNUT SOMATIC EMBRYOS |
| Authors: | V. Troch, N. Vermeir, S. Werbrouck, M.-C. Van Labeke |
| Keywords: | Aesculus hippocastanum, somatic embryogenesis, embryo maturation, osmoticum |
| Abstract:
Somatic embryogenesis could represent a way to clonally propagate mature elite trees of horse chestnut. However, while a number of protocols describe successfully the induction and proliferation of somatic embryos a low frequency of embryos converted successfully into plants. The objective of this research was to identify a maturation medium that could contribute to the outcome of more efficient germination results. Therefore, in a first approach the effects of sucrose 3 and 6%, and maltose 3%, were evaluated on somatic embryo maturation, in terms of subsequent somatic embryo germination. Carbon source and concentration had a marked influence on maturation, and subsequent germination of horse chestnut somatic embryos, with sucrose 6% promoting the highest frequency of germinated embryos larger than 1 cm. | |
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