ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
SOMATIC EMBRYOGENESIS INDUCTION IN DIFFERENT GENOTYPES OF COFFEA SPP. |
| Authors: | M.C. Simões-Costa, E. Carapuça , I.R. Moura |
| Keywords: | clonal propagation, coffee, plant growth regulators, plant tissue culture, somatic embryos |
| Abstract:
Cultures were established from leaf explants of different Coffea Arabica and C. arabica × C. canephora genotypes belonging to the Coffee Rusts Research Center (CIFC/IICT) greenhouse collection. Leaf explants were cultured for different periods of time (6 or 12 weeks) in solid induction medium containing SH salts, B5 vitamins, 5 μM 2,4-D and 20 BA μM. Explants were subsequently transferred to medium with the same composition but devoid of 2,4-D to promote embryo development. Embryogenic structures and calli were transferred to liquid media with half concentration of SH salts, casein hydrolysate and supplemented with different growth regulators in order to increase somatic embryos proliferation. Somatic embryogenesis was observed in all the studied genotypes. A longer period in the induction medium had no positive influence in the percentage of explants presenting embryogenic structures (which occurred in 100% of the explants in two of the studied genotypes). However, in some genotypes, the number of somatic embryos per explant was increased when they remained in culture for a longer period. Liquid medium favoured the proliferation of somatic embryos. Somatic embryos in different developmental stages are being used to develop vitrification-based cryopreservation protocols. | |
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