ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
MICROSHOOT ENCAPSULATION FOR COLD STORAGE, ACCLIMATIZATION, AND CLEAN UP FROM ARTHROPOD INFESTATIONS |
| Authors: | J.E. Preece, T.P. West |
| Keywords: | alginate, hardy hibiscus, hardening-off, micropropagation, preservation |
| Abstract:
Nodal segments (4 mm long) were excised from proliferating Stage II axillary shoot cultures of Hibiscus moscheutos and encapsulated in a sodium alginate gelled Driver and Kuniyuki Walnut (DKW) medium solidified with 50 μM CaCl2, then stored under refrigeration for at least four weeks in darkness. Encapsulated hibiscus nodes could be stored under refrigerated darkness for up to 1.5 years and retain viability. The longer the storage time, the slower the recovery to normal rates of shoot proliferation. During a mite infestation of proliferating hibiscus cultures, the incorporation of the insecticide acephate into the encapsulation medium during lab cleanup eradicated the mites. We were less successful incorporating the fungicide benomyl into the medium to eliminate fungal contamination introduced by the culture mites. After removal from dark refrigeration, if nodes were given 2-3 weeks of light pretreatment, they could be planted in a greenhouse in vermiculite and placed under mist. If planted 1 cm deep, all encapsulated nodes rooted and their axillary shoot elongated into plants. Anatomical comparisons were made among in vitro-produced leaves, leaves produced under mist on sprouting encapsulated nodes, and leaves from rooted cuttings in the greenhouse. The new leaves from the encapsulated nodes were intermediate in anatomy and epicuticular waxes. | |
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