ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
PRODUCTION OF MULTIPLE SHOOTS FROM CAROB TREE (CERATONIA SILIQUA) USING TISSUE CULTURE TECHNIQUE |
| Authors: | A.I. Mohamed, E.G. Elnour, S.A. Mahfouz |
| Keywords: | in vitro culture, cotyledon nodes, cytokinins, rooting, acclimatization |
| Abstract:
The aim of this study was to produce multiple shoots from cotyledons nodes of the seedlings of carob tree (Ceratonia siliqua L.) using MS medium supplemented with different concentrations of cytokinins, BAP (1, 2 and 3 mg/L), Kin (0.5, 1, 2 and 3 mg/L) and TDZ (0.05, 0.1 and 1 mg/L). The highest percentage of shoots production (91.67%) and the highest rate of shoot height (3.27 cm) were observed on MS medium containing 1.0 mg/L BAP. The highest shoot number (2.12 shoot per cotyledons nodes) was obtained when MS medium was supplemented with TDZ (1 mg/L). On the other hand, the lowest rates and percentages of multiple shoots formation were obtained when 1 or 3 mg/L Kin was added to the medium. This study also revealed that half-strength MS medium supplemented with IAA at 0.5 mg/L allowed the best rooting results. The obtained plants were easily adapted to normal conditions when grown in pots containing peat moss. | |
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