ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
USE OF ANTIOXIDANTS FOR ENHANCING FLAX MULTIPLICATION RATE IN TISSUE CULTURE |
| Authors: | D. Grauda, A. Miķelsone, I. Rashal |
| Keywords: | calli culture, ambiol, AV-153, ascorbic acid, triovit, regeneration |
| Abstract:
The aim of investigation was to develop a tissue culture approach that includes combination of a seed sterilisation method, the use of silver nitrate and antioxidants during different stages of calli induction, and shoot regeneration from flax accessions with low regeneration capacity. All used flax varieties (‘Vega 2’, ‘Lirina’, and ‘Rigaer B’) were excellent for calli culture formation (100% of explants form calli). Addition of AgNO3 to the seed germination medium promoted calli culture formation. Development of plants-regenerants was depended on the used 2,4-D concentration in calli induction medium, antioxidant, explant type (stem segments, leaves) and genotype but was not related to the calli weight. Antioxidants increased calli regeneration capacity. Plants-regenerants were obtained only from stem calli. | |
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