ISHS Acta Horticulturae 812: III International Symposium on Acclimatization and Establishment of Micropropagated Plants
IN VITRO ADAPTABILITY OF SOME ITALIAN OLIVE GENOTYPES |
| Authors: | M.E. Varlaro, T. Casacchia, B. Alfei, C. Briccoli Bati |
| Keywords: | Olea europaea L., olive micropropagation, media, OM, MSM |
| Abstract:
The micropropagation of some olive cultivars is today a well known technique, however, it does not exist a universal protocol for nursery productions, and the introduction and the establishment in vitro of many important olive varieties is still difficult. The aim of this work was, therefore, to study the adaptability of several Italian genotypes of Olea europaea L. in order to perform the propagation by mean of this technique. Explants sterilization and in vitro establishment of shoot cultures were investigated using two sterilization methods and two basal media, OM (Rugini, 1984) and modified MS (Murashige and Skoog, 1962) both added with mannitol as carbon source and with zeatin as cytokinin. For all the tested cultivars, a better sterilization was achieved using a mixture of sodium hypochlorite and hydrochloric acid instead of mercury chloride. The in vitro proliferation capacity of the different genotypes studied showed considerable differences regardless the media employed. The same cultivar showed a different behaviour in the two media and a promising improvement in shoot multiplication was generally obtained in the MS modified medium. | |
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