ISHS Acta Horticulturae 810: IX International Vaccinium Symposium
IN VITRO MULTIPLICATION OF HIGHBUSH BLUEBERRY (VACCINIUM CORYMBOSUM L.) CULTIVARS |
| Authors: | J. Sedlak, F. Paprstein |
| Keywords: | explant, micropropagation, zeatin, sterilization, rooting |
| Abstract:
The purpose of this study was to develop an efficient in vitro multiplication system for highbush blueberry cultivars ´Blueray´, ´Bluecrop´ and ´Berkeley´. The three genotypes were successfully established in vitro using 0.15% mercuric chloride as a sterilization solution. Anderson’s rhododendron medium (AN), half-strength Murashige and Skoog medium (half-MS) and McCown woody plant medium (WPM) containing the cytokinin zeatin in concentrations 0.5, 1 or 2 mg L-1 were tested. Anderson's rhododendron medium and McCown woody plant medium proved to be suitable for cultivation of selected highbush blueberry cultivars in vitro. The highest multiplication 4.8 ± 0.2 was noted for ´Berkeley´ on McCown woody plant medium with zeatin (2 mg L-1). The lowest multiplication was noted for all cultivars on half-strength Murashige and Skoog medium. | |
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