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| Authors: | M. Stepanovic, P. Vuksa, I. Potocnik, S. Milijasevic, E. Rekanovic, B. Todorovic |
| Keywords: | early blight, tomato, inhibition, toxicity, in vitro |
Abstract:
Six isolates of Alternaria solani, the causal agent of tomato early blight, collected from various tomato-growing areas in Serbia, were tested in vitro for sensitivity to six fungicides: chlorothalonil, copper-oxychloride, mancozeb, difenoconazole, iprodione and prochloraz.
The effective fungicide concentrations that inhibited mycelial growth by 50% (EC50) and their inhibitory activity were determined.
The tests showed that copper-oxychloride and chlorothalonil had the lowest toxicity to all investigated isolates.
Their respective EC50 values ranged between 109.72 - 173.32 mg L-1 and 40.47 - 162.94 mg L-1. Mancozeb was moderately toxic to all A. solani isolates with EC50 values from 9.79 to 24.35 mg L-1. The toxicity of iprodione, difenoconazole and prochloraz to all isolates was extremly high, their EC50 values ranging between 0.40– 0.80 mg L-1, 0.09 – 0.33 mg L-1 and 0.03 – 0.11 mg L-1, respectively.
The best inhibition of mycelial growth, exceeding 89.52%, was recorded in iprodione, prochloraz and difenoconazole treatments at a concentration of 100 mg L-1. The foregoing fungicides also had the highest inhibition at a concentration of 1 mg L-1, the respective values being 57.06 – 68.59%, 76.15 – 81.55% and 69.88 – 81.86%. Copper-oxychloride, chlorothalonil and mancozeb caused minor inhibition levels at the concentration of 1 mg L-1 (up to 26.98%). Among the fungicides investigated, iprodione, prochloraz and difenoconazole were the most toxic, while copper-oxychloride was the least toxic to all A. solani isolates.
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