ISHS Acta Horticulturae 808: II International Symposium on Tomato Diseases
OCCURRENCE OF BACTERIAL CANKER OF TOMATOES IN SOUTHERN SERBIA |
| Authors: | S. Milijasevic, B. Todorovic, E. Rekanovic, M. Stepanovic |
| Keywords: | Clavibacter michiganensis subsp. michiganensis, identification, PCR |
| Abstract:
Wilt and canker symptoms were first recorded on tomato cvs. Belle and Magnus in greenhouses owned by small-scale growers in two villages in southern Serbia in June 2006. In order to identify the causal agent, conventional identification methods (isolation, pathogenicity and bacteriological determinative tests) and polymerase chain reaction were used. The results of physiological and biochemical tests of the bacterium were as follows: Gram positive; oxidative metabolism of glucose; catalase positive; oxidase negative; levan negative; aesculin hydrolysis positive; starch hydrolysis negative; casein hydrolysis negative; H2S produced from peptone; acid produced aerobically from mannose but not from mannitol; and sodium acetate used as a carbon source. According to the morphological characteristics on nonselective media Nutrient Agar (NA) and Nutrient Broth Yeast extract agar (NBY), and selective media (D2ANX, mSCM and mCNS), biochemical characteristics, hypersensitive response in four-o’clock plant leaves and pathogenicity test on tomato seedlings, the investigated strains were identified as C. michiganensis subsp. michiganensis. To confirm the identity of isolated strains two PCR protocols were used. Amplification of DNA fragments of 614 bp and DNA fragments of 270 bp confirmed that the investigated strains belonged to C. michiganensis subsp. michiganensis. | |
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