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ISHS Acta Horticulturae 808: II International Symposium on Tomato Diseases

OCCURRENCE OF RALSTONIA SOLANACEARUM BIOVAR 2 ON TOMATO, WEEDS AND IRRIGATION WATER IN TURKEY

Authors:   N. Ustun, M. Ozakman, A. Karahan
Keywords:   tomato, bacterial wilt, irrigation water, weed hosts
Abstract:
The study was carried out to determine the causal agent of disease showing wilting symptoms on tomato in August of 2006 in some fields of Batakova locality of Canakkale province of Aegean Region of Turkey. Besides that irrigation water was tested for the presence of the pathogen and some of the potential weed hosts were also investigated. Samples taken from wilting tomato plants, canal water used for irrigation and potential weed hosts were tested by isolation onto modified SMSA medium. Isolated bacteria were identified by biochemical, immunofluorescence (IF), Real-time PCR and pathogenicity tests as Ralstonia solanacearum biovar 2. Fourteen out of sixteen water samples taken from the canal water in September 2006 were contaminated and population limits varied between 1x10 2-4.6 x10 2 cfu/ml. Several potential weed hosts were present in the fields but up to now the pathogen was isolated only from Solanum nigrum, S. alatum, Portulaca oleracea and Datura stramonium. Main weed host, S. dulcamara, was not found in or around water canal harboring the pathogen.

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