ISHS Acta Horticulturae 808: II International Symposium on Tomato Diseases
STUDY OF TOMATO SPOTTED WILT VIRUS (TSWV) ON TOMATOES IN MARKAZI PROVINCE, IRAN |
| Authors: | A. Esmaailifar, GH. Mossahebi, M. Mohammad |
| Keywords: | DAS-DIBA, DAS-ELISA, SSEM, Tomato Spotted Wilt Vims, tomato |
| Abstract:
Tomato spotted wilt virus (TSWV) was detected in tomato leaf and fruit samples collected from Arak region in Markazi province using indicator test plants including Petunia hybrida, Nicotiana glutinosa, N. tabacum cv. Samsun NN, N. clevelandii and N. benthamiana and serological tests. Small brownish local necrotic lesions appeared on P. hybrida leaves 2-4 days post-inoculation. Systemic symptoms included concentric ring spots on leaves, stem necrosis, wilting and tissue collapse of plants 7-10 days following the inoculation. Tomato samples were infected with TSWV were tested using ELISA, DIBA and SSEM. TSWV host range specificity and symptom expression were tested on Capsicum annuum L., Chenopodium amaranticolor L., Citrullus vulgaris L., Cucumis melo var. inodorus, C. melo var. reticuhitus, C. sativus L., Lycopersicon esculentum Mill., Phaseolus vulgaris L., Solanum melongena L. and S. tuberosum. Typical symptoms on these plants included concentric ring spots, chlorosis, vein clearing, tissue necrosis, stunting and local lesion formation. Antiserum prepared against a partially purified TSWV preparation cross-reacted with TSWV-infected tomato samples. | |
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