ISHS Acta Horticulturae 808: II International Symposium on Tomato Diseases
FURTHER SPREADING OF TOMATO YELLOW LEAF CURL SARDINIA VIRUS IN SOUTHERN ITALY |
| Authors: | S. Comes, R. Pacella, A. Fanigliulo, A. Crescenzi |
| Keywords: | tomato, TYLCSV, Tomato Yellow Leaf Curl Sardinia Virus, Italy |
| Abstract:
During autumn 2005-2006 a serious TYLC disease was observed in protected tomato crops in the Ionic coast of Basilicata region, with incidence of infections up to 100% under plastic tunnels. Symptoms consisted in marginal leaf yellowing and curling, plant stunting and flower abortion. Plants, tested for the presence of TYLCSV and/or TYLCV by PCR-RFLP of the 580 bp long amplicon obtained with the TY-1/TY-2 primer pair, resulted infected by a TYLCSV isolate. The genome of this isolate, named TYLCSV-N, was cloned, sequenced and compared with other Begomoviruses. It consists of one circular ssDNA molecule of 2773 nts with two ORFs in the virion sense and four in the complementary sense, separated by an intergenic region of 302 nts. In sequence comparison with other Geminiviruses, TYLCSV-N was most closely related to a TYLCSV from Sicily (99%) and one from Israel (98%), showing only low identity (91%) with one from Spain, all with a monopartite genome. The TYLCSV-N isolate could have reached the Ionic coast of Basilicata from Sicily through Calabria region, by means of viruliferous B. tabaci or infected plants. | |
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