ISHS Acta Horticulturae 808: II International Symposium on Tomato Diseases
ELIMINATION OF A DNA VIRUS ASSOCIATED WITH YELLOW LEAF CURL DISEASE IN TOMATO USING AN ELECTROTHERAPY TECHNIQUE |
| Authors: | M. Falah, J. Mozafari, N. Sokhandan Bashir, M. Hashemi |
| Keywords: | virus elimination, DNA virus, TYLCV, electrotherapy, PCR, tomato tissue culture |
| Abstract:
Tomato yellow leaf curl virus (TYLCV), a circular ssDNA Begomovirus of family Geminiviridae, causes serious losses in many plants such as tomato and ornamental plants. Possibility of eliminating DNA viruses from plants was investigated using TYLCV-tomato system. Infected plants collected from southern Iran were identified by PCR using a pair of specific primers amplifying a 670 bp DNA fragment. Detected virus was transmitted to healthy plants with insect vector Bemisa tabaci. TYLCV infected plants were used for electrotherapy, four weeks post inoculation. Nodal segments of 4-5 cm long carrying auxiliary buds were exposed to different electric currents (15, 25 and 35 mA°) for periods of 5, 10 and 15 minutes respectively. Buds were excised after electrotherapy and grown on a half strength MS medium. Six weeks old in vitro plantlets were evaluated for TYLCV infection by PCR, indicating that electrotherapy successfully eliminated the DNA virus TYLCV from tomato plants. Significant differences were observed among electrotherapy treatments in eradicating TYLCV. The electric current of 35 mA° for 10 minutes was the most effective electrotherapy treatment for genotype KC315022, while this was 35 mA° for 15 minutes for genotype KC315074. Our results showed that electrotherapy can be used as an effective technique to eliminate DNA viruses from plants, in addition to RNA viruses. | |
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