ISHS Acta Horticulturae 806: International Symposium on Underutilized Plants for Food Security, Nutrition, Income and Sustainable Development
TOWARDS MOLECULAR BREEDING OF PIGEONPEA (CAJANUS CAJAN (L.) MILLSP.): A CASE FOR INCREASED PRODUCTION IN AFRICA |
| Authors: | D.A. Odeny, C. Gebhardt |
| Keywords: | semi-arid tropics, microsatellites, simple sequence repeats (SSRs), EST-SSRs, orphan crops, underutilized species |
| Abstract:
Simple sequence repeats (SSRs) were developed in pigeonpea by using two techniques: screening an enriched pigeonpea genomic library and transfer of SSRs from soybean (Glycine max L.) genome. Primers were designed for 113 pigeonpea genomic SSRs, 73 of which amplified interpretable bands. Thirty-five of the primers revealed polymorphism among 24 pigeonpea breeding lines. The number of alleles detected ranged from two to six with a total of 110 alleles and an average of 3.1 alleles per locus. TG class of repeats and GAA were the most abundant di-nucleotide and tri-nucleotide repeats respectively. Additional 220 soybean primers were tested in pigeonpea, 39 of which amplified interpretable bands. Microsatellites provide a powerful tool for genomic studies and are recommended for systematic fingerprinting of other underutilized species. | |
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