ISHS Acta Horticulturae 798: III International Symposium on Fig
FIG MOSAIC DISEASE: ASSOCIATED PROTEIN AND CDNA |
| Authors: | L. Serrano, A. Benito, V. Medina, M.A. Achón |
| Keywords: | virus, purification, protein, RT-PCR, sequencing |
| Abstract:
Fig mosaic (FM) is the most widespread disease affecting the majority of fig varieties worldwide. FM belongs to a disease group associated with to the presence of double membrane-bound bodies (DMBs) in the cytoplasm of infected cells and transmitted by eryophyid mites. In accordance with these diseases, it has been pointed out that the causal agent of FM could be a virus, and it has been related with uncharacterisated Potyvirus and Carlavirus. Virus purification procedures for both isometric and rod-shaped particles, RNA analysis, and RT-PCR have been evaluated for FM-affected fig leaves. Rod-shaped virus-like particles and isometric particles back-ground were observed in the majority of purified virus preparations. The procedure for Tenuivirus-like particles purification rendered the highest yield of particles. Four RNA fragments ranging from 700 to 5000 nt were detected, and six specific cDNA products ranging from 600 to 3000 pb were obtained. After cloning and sequencing these products we constated that they did not show any significant match in comparison to other viral and non-viral sequences available in the GenBank. The translation product of 700 pb cDNA contained the capsid protein (CP) motifs, and it is related to the CP of Cripavirus genus (Fam. Dicistroviridae). Three proteins associated with FM were detected. | |
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