ISHS Acta Horticulturae 795: V International Cherry Symposium
DETERMINATION OF IN VITRO PROPAGATION TECHNIQUES FOR SOME CLONAL CHERRY ROOTSTOCKS |
| Authors: | A. Fidancı, M. Burak, B. Erenoglu, M.E. Akçay |
| Keywords: | Prunus avium, micropropagation, rooting, acclimation, vitrification |
| Abstract:
In this study, rapid in vitro propagation techniques were determined for some clonal cherry rootstocks (‘Gisela 5’, ‘MaxMa 14’ and ‘Tabel Edabriz’). The most appropriate time for taking explants is from the end of April to the beginning of June. Taking explants earlier caused a high level of contamination, whereas browning increased and growth and multiplication rates were reduced, for those collected later. Shoot tip and axillary buds were used as explant sources. Sterilization, culture establishment, multiplication, rooting and acclimation were investigated. In the culture establishment stages, contamination was the only problem, whereas the most important problem in the multiplication stage was vitrification. In the rooting stage, MS + 1 mg/L IBA gave 95 to 100% rooting in three weeks. The rooted plantlets were transferred to vials or small plastic pots containing a peat moss + perlite 1:1 (v/v) medium and had a high rate of survivability in the acclimatization stage. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
